Technical characteristics
- BrandAGLYCO
- Country of manufactureChina
- NoNo
Description
Heparinase I (heparin lyase I) cleaves heparin and to a lesser extent heparan sulfate (ratio approx. 3:1).
Properties
Molecular weight: 42.8 kDa
Isoelectric point: 9.3 – 9.5
pH optimum for activity: 7.4
pH range for activity: 4 – 9
Optimal temperature range: 20 ℃ – 37 ℃
Purity: ≥ 98 % by reversed phase HPLC analysis
Protein impurities: < 0.1%
Specific Activity: > 400 IU/mg (max. 417 IU/mg)
Concentration: 10 IU/mL
Unit Definition: One international unit (IU) of heparinase I is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from porcine mucosal heparin per minute at 35 ℃ and pH 7.0.
Stability: Expiration of heparinase I is 12 months from manufacturing date, frozen at -20 ℃ in PBS.
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Heparinase I